- Page Contents:
- Applications
- Features
- What you provide
- What is included
- Advantages
- Schedule
- Notice
Construction of Adenovirus Vectors Containing Two Expression Cassettes in the E1- and E3- Regions
Applications:
- Vectors containing two genes of interest under the control of their own promoter
- Vectors containing your gene of interest in the E1 region and a reporter gene in the E3 region or vice-versa
- Vectors containing inducible expression systems
- Vectors expressing a regulator and a regulated gene
- Vectors expressing two genes involved in the same pathway
Features:
- AdenoQuick™
- Ad5 backbone
- E1 deletion 3.1 kb (bp 354-3503)
- E3 deletion: 2.7 kb (bp 28133-30818)
- Total insertion capacity of 7.6 kb
You provide:
- Two expression cassettes including promoters, coding sequences and polyadenylation signals
- Restriction maps, or preferably the sequences of your transgenes
The service includes:
- Construction of shuttle vectors containing your expression cassettes
- Construction of a cosmid containing the entire sequence of your recombinant virus
- Virus recovery by transfecting the DNA into helper cells
- Virus clone isolation by plaque assay
- Confirmation of virus identity at the DNA level
You can customize your request: for example, you can decide to construct the shuttle vector yourself and send it to us for virus recovery. Another popular option is to allow O.D.260 to construct the cosmid which contains the entire sequence of your recombinant adenovirus and you will recover the virus yourself.
TopAdvantages of using our service:
- State-of-the-art AdenoQuick™ system
- Fast turn-around
- Low cost
Minimum time to completion:
| Shuttle vector construction | 4 days |
| Cosmid construction | 4 days |
| Virus recovery (from transfection to virus plaque isolation) | 6 days |
| Confirmation of virus genome | 5 days |
| Total full service | 3 weeks |
An Important Note on Handling Adenovirus:
Adenovirus is an infectious respiratory virus and its use as a genetic vector requires the use of adequate containment equipment and practices. Biosafety Level 2 (BL2) is appropriate for most customers; however, for some, use of BL3 or higher level containment facility may be required. PLEASE NOTE, O.D.260 Inc. will not carry out projects that require BL3 (or above) containment.
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