AdenoQuick13.1 |
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* E1/E3-deleted adenovirus vectors * Dicistronic (a.k.a. bipartite) vectors: Transgene insertion in the E1 region and E3 region (max. 7.5 kb cargo capacity) * 2.7 kb E3 deletion * AdenoQuick cloning system |
| Ordering Information | Resources |
Related products |
| Cat # QR-03 | AdenoQuick Cloning system (overview & details) |
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| Qty: 10 reactions (SfiI-digested plasmid DNA) | AdenoQuick Manual (PDF) |
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Map & Features (PDF) |
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| Pricing and order form | Sequence (request by email) |
Description
AdenoQuick13.1 is a 36.7 kb plasmid that contains the sequences encompassing bp 3504-right end (9.8–100 mu) of the Ad5 genome. The two SfiI sites naturally present in WT Ad5 DNA were mutated by substituting A for G and C at positions 16291 and 16294 in the Ad5 genome, and C and G for respectively G and C at positions 23001 and 23004 in the Ad5 genome, introducing silent mutations in the adenovirus pVII and DNA-binding protein coding sequences. Two pairs of SfiI sites that allow for directional cloning replace the E1 and E3 regions. The size of the E3 region deletion is 2.7 kb. The right ITR is flanked by PacI and SwaI sites. AdenoQuick13.1 is used in combination with the shuttle vectors pE1.2 and pE3.1 to construct bipartite replication-deficient adenoviruses containing transgenes in place of the E1 and E3 regions. The maximum combined transgene capacity is 7.5 kb. |