pAd330 |
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* Conditionally replicative adenoviruses (CRAds) * Promoter insertion in front of the E1a TATA box (max. 4.6 kb) * 2.7 kb E3 deletion * AdenoQuick cloning system |
| Ordering Information | Resources |
Related products |
| Cat # OP-03 | AdenoQuick Cloning system (overview & details) |
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| Qty: 10 reactions (SfiI-digested plasmid DNA) | ||
Map & Features (PDF) |
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| Pricing and order form | Sequence (request by email) |
Description
pAd330 is a 39 kb plasmid that contains the sequences encompassing bp 466-right end (1.3–100 mu) of the Ad5 genome, with a 2.7 kb deletion in the E3 region. The two SfiI sites naturally present in WT Ad5 DNA were mutated by substituting A for G and C at positions 16291 and 16294 in the Ad5 genome, and C and G for respectively G and C at positions 23001 and 23004 in the Ad5 genome, introducing silent mutations in the adenovirus pVII and DNA-binding protein coding sequences. A pair of SfiI sites that allow for directional cloning replaces the E1 promoter. The right ITR is flanked by PacI and SwaI sites. pAd330 is used in combination with the shuttle vector pE1.1 to construct conditionally replicative adenoviruses (CrAds) containing a heterologous promoter in front of the E1a TATA box. The maximum capacity for insertion is 4.6 kb. |