pE1.1

* Shuttle vector for inserting heterologous sequences in place of the E1 region of the Ad5 genome

* E1-substituted adenovirus vectors with E3 and/or E4 regions WT or deleted

* Conditionally replicative adenovirus vectors (CRAds)

* AdenoQuick cloning system

pE1.1 is a shuttle vector designed for inserting expression cassettes in place of the E1 region of the Ad5 genome, in combination with the AdenoQuick1.1, AdenoQuick1.2, pAd361 and pAd362 plasmids (construction of first- and second-generation adenovirus vectors). It contains the first 353 base pairs (map unit 0-1) from the Ad5 genome (including the left ITR and packaging signal), preceded by PacI and SwaI sites, and followed by a multiple cloning site. Expression cassettes inserted into this site should contain a promoter, coding sequence and a polyA signal. The sequences encompassing the kanamycin-resistance gene, the λ cos site, the adenovirus 0-1 map units and the multiple cloning site are flanked by two sets of restriction sites AlwNI, BstAPI, DraIII and PflMI which generate incompatible and non-symmetrical sticky ends. pE1.1 can also be used in combination with vectors pAd329 and pAd330 to construct conditionally replicative adenoviruses (CRAds) containing a heterologous promoter in front of the E1a TATA box.