pE3-CMV

* Shuttle vector for inserting CMV-driven expression cassettes in place of the E3 region of the Ad5 genome

* E1- and E3- substituted adenovirus vectors with E4 region WT or deleted (bipartite, dicistronic adenovirus vectors)

* "Armed" conditionally replicative adenovirus vectors (CRAds)

* AdenoQuick1.0 cloning system

pE3-CMV is a shuttle vector designed for inserting CMV-driven expression cassettes in place of the E3 region of the Ad5 genome, in combination with the pE1.2 and AdenoQuick13.1 plasmids.  It contains a CMV promoter - multiple cloning site (MCS) – bovine growth hormone (bGH) poly(A) signal adjacent to a 178 bp-long λ cos site.  The sequences encompassing the cos site and the CMV-bGHp(A) expression cassette are flanked by two sets of restriction sites AlwNI, BstAPI, DraIII and PflMI which generate non-symmetrical sticky ends useful for directional cloning.  pE3-CMV can also be used in combination with plasmids pAd328 and pE1.2 to construct conditionally replicative adenoviruses (CrAds) containing a heterologous promoter in front of the E1a TATA box, and a transgene in place of the E3 region.  It can be used also in combination with plasmids pAd363 and pE1.2 to construct ΔE1/E3/E4 vectors with maximum 8.7 kb cargo capacity.