pE3.1

* Shuttle vector for inserting heterologous sequences in place of the E3 region of the Ad5 genome

* E1- and E3- substituted adenovirus vectors with E4 region WT or deleted (bipartite, dicistronic adenovirus vectors)

* "Armed" conditionally replicative adenovirus vectors (CRAds)

* AdenoQuick1.0 cloning system

pE3.1 is a shuttle vector designed for inserting expression cassettes in place of the E3 region of the Ad5 genome, in combination with the pE1.2 and AdenoQuick13.1 or pAd363 plasmids. It contains a multiple cloning site (MCS) adjacent to a 178 bp-long λ cos site. Expression cassettes inserted into the MCS should contain a promoter, coding sequence and a polyA signal. The sequences encompassing the cos site and the MCS are flanked by two sets of restriction sites AlwNI, BstAPI, DraIII and PflMI which generate non-symmetrical sticky ends useful for directional cloning. pE3.1 can also be used in combination with plasmids pAd328 and pE1.2 to construct "armed" conditionally replicative adenoviruses (CRAds) containing a heterologous promoter in front of the E1a TATA box, and a transgene in place of the E3 region. It can be used also in combination with plasmids pAd363 and pE1.2 to construct ΔE1/E3/E4 vectors with maximum 8.7 kb cargo capacity.