pZAP1.1 |
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* Shuttle vector for inserting heterologous sequences in place of the E1 region of the Ad5 genome ("first-generation" vectors) * E3 and/or E4 regions WT or deleted * Ad5 or Ad35 knob * AdenoZap cloning system |
| Ordering Information | Resources |
Related products |
| Cat # ZP-01 | AdenoZAP Cloning system . (overview & details) |
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| Qty: 20 ug | AdenoZAP Manual (PDF) |
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Map & Features (PDF) |
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| Pricing and order form | Sequence (request by email) |
Description
pZAP1.1 is a 3.5 kb shuttle vector designed for inserting expression cassettes in place of the E1 region of the Ad5 genome, in combination with RightZAP1.1 (WT E3), RightZAP1.2 (ΔE3), RightZap1.3 (ΔE3 + Ad35 knob) and other vectors from the AdenoZAP1.X cloning system. It contains a multiple cloning site downstream from the left ITR and packaging signal of the Ad5 genome. Expression cassettes inserted into this site should contain a promoter, cDNA, and polyA signal. The left arm DNA (which corresponds to the left ITR, packaging signal and expression cassette) can be excised from the vector with either PacI or SwaI on one side, and either PflMI, DraIII, SfiI, BstAPI or AlwNI on the other side. |