pZAP2.1 |
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* Shuttle vector for inserting heterologous sequences in place of the E1 region of the Ad5 genome ("first-generation" vectors) * E3 region WT or deleted * AdenoZap2.0 cloning system |
| Ordering Information | Resources |
Related products |
| Cat # ZP-02 | AdenoZAP Cloning system . (overview & details) |
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| Qty: 20 ug | AdenoZAP Manual (PDF) |
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Map & Features (PDF) |
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| Pricing and order form | Sequence (request by email) |
Description
pZAP2.1 is a shuttle vector designed for inserting expression cassettes in place of the E1 region of the Ad5 genome, in conjunction with the AdenoZAP2.1 system. It contains a multiple cloning site located downstream from the first map unit (mu) of the Ad5 genome. Expression cassettes should contain promoter-cDNA-polyA signal. The left arm DNA (which includes the left ITR, packaging signal and expression cassette) can be excised from the vector with either PacI or SwaI on one side, and I-SceI on the other side. |