Additional Component Details
Components:
RightZap1.1 DNA Cat # ZR-01
The RightZap1.1 DNA is a 32.5 kb linear DNA fragment that encompasses bp 3504-right end of the Ad5 genome. The E3 region is intact. The DNA was purified from a cosmid. It is used in combination with the shuttle vector pZap1.1 to construct replication-deficient adenoviruses containing transgenes in place of the E1 region of the Ad5 genome. The maximum transgene capacity of the resulting virus is 5.2 kb.
| Contents | Cat. # | Quantity | Pricing |
| RightZap1.1 (WT E3) | ZR-01 | 20 µg (10 constructions) | $400.00 |
RightZap1.2 DNA Cat # ZR-02
The RightZap1.2 DNA is a 29.8 kb linear DNA fragment that encompasses bp 3504-right end of the Ad5 genome. The E3 region is deleted (2.7 kb). The DNA was purified from a cosmid. It is used in combination with the shuttle vector pZap1.1 to construct replication-deficient adenoviruses containing transgenes in place of the E1 region of the Ad5 genome. The maximum transgene capacity of the resulting virus is 7.9 kb.
| Contents | Cat. # | Quantity | Pricing |
| RightZap1.2 (ΔE3) | ZR-02 | 20 µg (10 constructions) | $400.00 |
RightZap2.1 DNA Cat # ZR-03
The RightZap2.1 DNA is a 32.5 kb linear DNA fragment that encompasses bp 3504-right end of the Ad5 genome. The E3 region is intact. The DNA was purified from a cosmid. It is used in combination with the shuttle vector pZap2.1 to construct replication-deficient adenoviruses containing transgenes in place of the E1 region of the Ad5 genome. The maximum transgene capacity of the resulting virus is 5.2 kb.
| Contents | Cat. # | Quantity | Pricing |
| RightZap2.1 (WT E3) | ZR-03 | 20 µg (10 constructions) | $400.00 |
RightZap2.2 DNA Cat # ZR-04
The RightZap2.2 DNA is a 29.8 kb linear DNA fragment that encompasses bp 3504-right end of the Ad5 genome. The E3 region is deleted (2.7 kb). The DNA was purified from a cosmid. It is used in combination with the shuttle vector pZap2.1 to construct replication-deficient adenoviruses containing transgenes in place of the E1 region of the Ad5 genome. The maximum transgene capacity of the resulting virus is 7.9 kb.
| Contents | Cat. # | Quantity | Pricing |
| RightZap2.2 (Δ E3) | ZR-04 | 20 µg (10 constructions) | $400.00 |
pZap1.1 Cat # ZP-01
pZap1.1 is a 3.5 kb shuttle vector designed for inserting expression cassettes in place of the E1 region of the Ad5 genome, in combination with the AdenoZap1.x system. It contains a multiple cloning site downstream from the first map unit of the Ad5 genome. Expression cassettes inserted into this site should contain promoter, cDNA, and polyA signal. The left arm DNA (which corresponds to the left ITR, packaging signal and expression cassette) can be excised from the vector with either PacI or SwaI on the left side, and either PflMI, DraIII, SfiI, BstAPI or AlwNI on the right side.
| Contents | Cat. # | Quantity | Pricing |
| pZap1.1 | ZP-01 | 20 µg | $200.00 |
pZap2.1 Cat # ZP-02
pZap2.1 is a 3.5 kb shuttle vector designed for inserting expression cassettes in place of the E1 region of the Ad5 genome, in combination with the AdenoZap2.x system. It contains a multiple cloning site downstream from the first map unit of the Ad5 genome. Expression cassettes inserted into this site should contain promoter, cDNA, and polyA signal. The left arm DNA (which corresponds to the left ITR, packaging signal and expression cassette) can be excised from the vector with either PacI or SwaI on the left side, and I-SceI on the right side.
| Contents | Cat. # | Quantity | Pricing |
| pZap2.1 | ZR-02 | 20 µg | $200.00 |
Control ΔE1 Left Arm (Sfi) Cat # ZL-01
The Control ΔE1 Left Arm (Sfi) is a 431 bp-long PacI-DraIII DNA fragment isolated from pZap1.1. It contains the left ITR, packaging signal and the multiple cloning site. This DNA fragment can be used to control the efficiency of the ligation with RightZap1.1 and RightZap1.2. By transfecting the ligation mixture into helper cells (e.g. 293 cells), you will be able to construct an E1-deleted adenovirus without expression cassette, which will be useful as negative control in your future experiments. Enough material is provided to perform this control ligation twice.
| Contents | Cat. # | Quantity | Pricing |
| Control ΔE1 Left Arm (Sfi) | ZL-01 | 100 ng | $150.00 |
Control ΔE1 Left Arm (Sce) Cat # ZL-02
The Control ΔE1 Left Arm (Sce) is a 453 bp-long SwaI-SceI DNA fragment isolated from pZap2.1. It contains the left ITR, packaging signal and the multiple cloning site. This DNA fragment can be used to control the efficiency of the ligation with RightZap2.1 and RightZap2.2. By transfecting the ligation mixture into helper cells (e.g. 293 cells), you will be able to construct an E1-deleted adenovirus without expression cassette, which will be useful as negative control in your future experiments. Enough material is provided to perform this control ligation twice.
| Contents | Cat. # | Quantity | Pricing |
| Control ΔE1 Left Arm (Sce) | ZL-02 | 100 ng | $150.00 |
Control Adβgal DNA Cat # ZC-01
The Control Adβgal DNA is a plasmid-isolated adenovirus DNA that contains a CMV-lacZ expression cassette in place of the E1 region. It can be used as a control to monitor the efficiency of transfection and virus recovery. It will generate a βgalactosidase-expressing adenovirus useful for future experiments. Enough DNA is provided for 5 transfections.
| Contents | Cat. # | Quantity | Pricing |
| Control Adβgal DNA | ZC-01 | 10 µg | $150.00 |
Price List
| Product | Cat # | Unit Price |
| AdenoZap1.1 Kit | ZK-01 | $550.00 |
| AdenoZap1.2 Kit | ZK-02 | $550.00 |
| AdenoZap1-Twin Kit | ZK-03 | $850.00 |
| AdenoZap2.1 Kit | ZK-04 | $550.00 |
| AdenoZap2.2 Kit | ZK-05 | $550.00 |
| AdenoZap2-Twin Kit | ZK-06 | $850.00 |
| RightZap1.1 DNA | ZR-01 | $400.00 |
| RightZap1.2 DNA | ZR-02 | $400.00 |
| RightZap2.1 DNA | ZR-03 | $400.00 |
| RightZap2.2 DNA | ZR-04 | $400.00 |
| pZap1.1 | ZP-01 | $200.00 |
| pZap2.1 | ZP-02 | $200.00 |
| Control ΔE1 Left Arm (Sfi) | ZL-01 | $150.00 |
| Control ΔE1 Left Arm (Sce) | ZL-02 | $150.00 |
| Adβgal DNA | ZC-01 | $150.00 |