- Page Contents:
- Applications
- What you provide
- What is included
- Advantages
- Schedule
- Notice
Virus Clone Isolation by Plaque Assay
It is good virological procedure to purify a recombinant virus by plaque assays, even if the virus was generated from a plasmid or cosmid isolated from a single E. coli clone. A recombinant adenovirus can prove unstable if the expression cassette it carries exceeds the maximal transgene capacity of the vector, or if it encodes a protein that is toxic for the helper cells or interferes with viral replication.
Applications:
- Isolation of viral clones during virus construction, prior to large-scale virus amplification
- Isolation of clones of a virus that might reveal unstable
- Isolation of clones from a virus suspension that contains replication-competent adenovirus (RCA)
You provide:
- A virus suspension
The service includes:
- Infection of monolayers of helper cells with a serial dilution of your virus – agar overlay
- Isolation of 10 plaques
- Small-scale amplification of each plaque
- Confirmation of virus identity at the DNA level (restriction analysis, PCR or Southern blot) or protein expression (Western-blot, RT-PCR).
Advantages of using our services:
- Short turn-around
- Reliability
- Cost-effective
Minimum time required:
- 2 weeks
An Important Note on Handling Adenovirus:
Adenovirus is an infectious respiratory virus and its use as a genetic vector requires the use of adequate containment equipment and practices. Biosafety Level 2 (BL2) is appropriate for most customers; however, for some, use of BL3 or higher level containment facility may be required. PLEASE NOTE, O.D.260 Inc. will not carry out projects that require BL3 (or above) containment.