- Page Contents:
- Applications
- What you provide
- What is included
- Advantages
- Schedule
- Notice
Titration of Adenovirus Preparation
We offer three methods for determining the infectious titer of an adenovirus suspension.
- The plaque assay is the most sensitive method for determining the concentration of infectious viral particles in a suspension. A cell monolayer is infected with a serial dilution of the virus suspension to be titrated, and covered with agar. Plaques are counted generally from 7 to 12 days after infection.
- The TCID50 method
- Immunostaining
Applications:
- Determination of the number of infectious units (IU) in a crude or purified virus suspension
You provide:
- A virus suspension
The service includes:
- Infection of monolayers of helper cells with a serial dilution of your virus and agar overlay (in triplicate)
- Determination of titer (pfu/ml)
Advantages of using our services:
- Short turn-around
- Optimized methods
- Cost-effective
Minimum time required:
- 12 days
An Important Note on Handling Adenovirus:
Adenovirus is an infectious respiratory virus and its use as a genetic vector requires the use of adequate containment equipment and practices. Biosafety Level 2 (BL2) is appropriate for most customers; however, for some, use of BL3 or higher level containment facility may be required. PLEASE NOTE, O.D.260 Inc. will not carry out projects that require BL3 (or above) containment.
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