Experts in Adenovirus Vectors

Cosmid Construction Kit-3

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Adenovirus cosmid construction via lambda packaging

Cosmid Construction Kit-3
New

Reagents for Adenovirus Cosmid Construction with AdenoQuick2.0 (electroporation)

  • Sufficient for constructing at least 10 vectors (that is only $14.50 per construct)
  • Reagents are automatically added to your order when you buy an AdenoQuick2.0 kit.
  • Contents:
    • E. coli OD101 electro-competent cells
    • Outgrowth medium for electro-competent cells
    • Amp/Kan antibiotic mix (#R-1007)
    • SfiI enzyme (#R-1008)
    • SfiI 10x buffer (#R-1009)
  • Storage: -20 ºC for SfiI enzyme and -70 ºC for electro-competent cells
$145.00
SKU
R-1003
Size
10 reactions
Description

The Cosmid Construction Kit -3  contains most of the reagents necessary to construct AdenoQuick2.0 cosmids via electroporation:

  • The SfiI restriction enzyme is used to digest the 4 shuttle plasmids before ligating them to each other.
  • OD101 electro-competent cells are used to construct and amplify adenovirus cosmid DNAs.
  • The outgrowth medium is used to culture the cells immediately after the electric shock.
  • The Amp/Kan antibiotic mix will be used to select for colonies containing the adenovirus cosmid.

The reagents that the user needs to provide are:

  • T4 DNA ligase
  • LB Lennox (liquid and petri dishes)

The SfiI restriction enzyme must be stored at - 20 ºC.  The electro-competent cells must be stored at - 70 ºC.

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